North Carolina State Research Articles

Heat-related fatalities in North Carolina 1999-2017.

Research shows the highest rates of occupational heat-related fatalities among farm laborers and among Black and Hispanic workers in North Carolina (NC). The Hispanic population and workforce in NC have grown substantially in the past 20 years. We describe the epidemiology of heat-related fatal injuries in the general population and among workers in NC. We reviewed North Carolina death records and records of the North Carolina Office of the Chief Medical Examiner to identify heat-related deaths (primaryInternational Classification of Diseases, Tenth Revisiondiagnosis code: X30 or T67.0-T67.9) that occurred between January 1, 1999, and December 31, 2017. Decedentage, sex, race, and ethnicity were extracted from both the death certificate and the medical examiner's report as well as determinations of whether the death occurred at work. In NC between 1999 and 2017, there were 225 deaths from heat-related injuries, and 25 occurred at work. The rates of occupational heat-related deaths were highest among males, workers of Hispanic ethnicity, workers of Black, multiple, or unknown race, and in workers aged 55-64. The highest rate of occupational heat-related deaths occurred in the agricultural industry. Since the last report (2001), the number of heat-related fatalities has increased, but fewer were identified as workplace fatalities. Rates of occupational heat-related deaths are highest among Hispanic workers. NC residents identifying as Black are disproportionately burdened by heat-related fatalities in general, with a wider apparent disparity in occupational deaths.

First Report of Bacterial Leaf Spot Disease on Sesame (Sesamum indicum L.) Caused by Pseudomonas syringae pv. sesami in North Carolina.

In July 2022, dark brown to black, angular, water-soaked lesions were observed on sesame leaves (Sesamum indicum L.) in a research plot established to assess yield potential for eight varieties at the North Carolina (NC) Sandhills Research Station (Chavez 2023). Symptoms were indicative of a bacterial leaf spot (BLS). At early flowering stage, leaf spots were present on scattered plants; varieties ES108, SS3301, and ES201 exhibited up to 75% disease prevalence, with lower frequency in ES103, S39, S4302, S3251, and S3276. Symptomatic leaves from 3-4 plants were collected on four different dates from July through September. A section of symptomatic tissue was excised and macerated in sterile deionized water (SDW). A 10 µL aliquot was streaked onto SPA medium (15 g sucrose, 5.0 g proteose peptone, 0.50 g MgSO4 7H2O, 0.25 g K2HPO4, 15 g agar per liter of SDW) and incubated at 28ºC. After 72 h, numerous, smooth, white-cream colored, convex-shaped, colonies were individually isolated. Five randomly selected isolates from the different collection dates, designated as AHP108-AHP111 and AHP116, were genotyped. The 16S rRNA, gyrB, rpoD, and gapA genes were sequenced (Heuer et al. 1997; Hwang et al. 2005) and deposited to NCBI (GenBank Accessions: P213467- PP213470; OQ628040-OQ628042; PP214983-PP214994; and PP255798). These five isolates shared 100% sequence identity for gyrB and rpoD. AHP108-AHP111 shared 100% sequence identity for 16S rRNA and gapA, with 99.7% and 90.8% identity, respectively, for AHP116. A phylogenetic tree was inferred from a maximum-likelihood analysis of concatenated gyrB, rpoD, and gapA sequences of the five isolates and the top 11 hts from a blastn search of the NCBI nucleotide database. Those hits included closely related sequences from Pseudomonas syringae pv. sesami type strains ICMP 763T and ICMP 7459T. Based on this phylogenetic analysis AHP108-AHP111 and AHP116 are P. syringae pv. sesami. Recent genomic analysis suggests this pathovar is part of P. amygdali (Gomila et al. 2017), but an official name change has not been proposed. Each of the five isolates were infiltrated into leaves of sesame varieties ES108, ES103, and S327, consistently resulting in similar symptoms. Thus, strain AHP116, as a representative, was used to fulfill Koch's postulates using five, 30-day-old potted sesame plants (var. S3301). Plants were spray-inoculated with a bacterial suspension of ~108 CFU/ml until runoff; plants were incubated in moist chambers 24 h pre and post inoculation at 28ºC with 80% relative humidity and a 12 h photoperiod. At 13 days post inoculation, symptoms resembling those on plants at the Sandhills Research Stations in 2022 were evident. Reisolated bacteria were confirmed to be AHP116 through 16S rRNA and gyrB amplification and sequencing. No symptoms were observed on the five water-inoculated plants. BLS of sesame has been reported in Asia and is thought to be seedborne (Firdous et al. 2009; Prathuangwong and Yowabutra 1997). To our knowledge, this is the first report of P. syringae pv. sesami causing BLS on sesame in North Carolina. Sesame cultivation in the state increased from approximately 2,000 acres in 2022 to 13,000 acres in 2023 and there is interest in cultivating sesame as a rotational and alternative crop because it requires minimal input costs. Potential outbreaks of BLS in this warm, humid region could negatively affect sesame production, where little is known about the economic impact of the disease.

A Man of Bad Reputation: The Murder of John Stephens and the Contested Landscape of North Carolina Reconstruction

A Man of Bad Reputation opens with John G. Lea’s confession that he had assassinated North Carolina State Senator John Walter Stephens in 1870. Lea gave his confession in 1919, nearly half a century after the murder. Stephens’s death, Drew A. Swanson contends, had profound repercussions. On the one hand, it “featured prominently in the US Congress’s investigation of Klan activities in the South,” which eventually led for the Enforcements Acts and the end of the first Ku Klux Klan. On the other hand, it sparked the Kirk-Holden War, which led to the impeachment of Governor William Woods Holden, his removal from office, the death of Reconstruction, and the rise of Jim Crow in North Carolina, “a new and even more effective form of white supremacy that used ‘soft’ terrorism in place of the Klan’s overt violence.” Swanson, currently Jack N. and Addie D. Averitt Distinguished Professor of Southern History at Georgia State University, offers a fascinating discussion of the life and death of Stephens, which also becomes a story about the North Carolina Piedmont, Reconstruction in North Carolina, and the ways in which people have remembered and misremembered this important period.

ARTISANS—Artificial Intelligence for Simulation of Advanced Nuclear Systems for Nuclear Fission Technology

The objective of this Technical Opinion Paper (TOP) is to provide an overview of the research topics in the ARTISANS (Artificial Intelligence for Simulation of Advanced Nuclear Systems) research group at the North Carolina State University. We will discuss the connections between our research with the key items outlined in the Virtual Special Issues (VSI) Nuclear Fission Technology (NFT) series. NFT is crucial for the global goal of decarbonization, but as outlined by the VSI framework, significant challenges remain that inhibit NFT profitability. Our research addresses some of these challenges, centered around improving the simulation of complex nuclear systems with Artificial Intelligence and Machine Learning (AI/ML). The ARTISANS group specializes in many aspects of applications of AI/ML to nuclear engineering, whose potential impact on NFT continues to grow as AI/ML technologies improve.

199 Early expansion and outcomes of a multilingual Latine review board for inclusive science

OBJECTIVES/GOALS: The NC Translational and Clinical Sciences (NC TraCS) Institute’s Latine Community Review Board (CRB) provides expertise to researchers seeking to culturally adapt interventions and improve language access in clinical and translational science. Early efforts aimed to expand CRB membership, build member capacity, and assess CRB impact. METHODS/STUDY POPULATION: Founded in 2021, the NC TraCS Latine CRB is an advisory group of natively Spanish-speaking, Latine North Carolinians who contract with research teams (RTs) to review Latine community-facing study materials (e.g., decision aids, scales, recruitment scripts) and provide feedback for improving or validating materials’ linguistic accuracy and cultural relevance. Bilingual staff facilitate members’ review of materials, use focus group methods to solicit feedback, and report key findings and suggested revisions to RTs. In 2023, we recruited new age-, gender-, and racially diverse members; and implemented process and impact evaluation, via (1) post surveys of RTs and (2) assessment of members’ experiences and visions for growth utilizing the Focused Conversation Method (Top-network, 2021). RESULTS/ANTICIPATED RESULTS: Since 2021, NC TraCS has conducted 11 Latine CRB review sessions to support 7 research studies. Current CRB members (n=8) report that (1) their work effectively supports Latine inclusion in research; (2) increased group size and diversity help ensure study materials are comprehensible to heterogenous Latine populations; and (3) an hourly member compensation rate equal to the hourly rate of TraCS’ staff accurately represents member contributions. Members also cited that they value open communication, continuous improvement, communication via email and group chat, periodic programming meetings, and RT updates on study outcomes. Post-session RT surveys to date (n=3) show that RTs would recommend this program to other researchers (pending survey responses from RTs this year will provide additional impact data). DISCUSSION/SIGNIFICANCE: Early data highlight the value of fairly compensated, bilingually coordinated input from diverse Latine community members for improving studies’ inclusion of heterogeneous, multilingual communities. Our approach can inform the development, growth, and continuous improvement of multilingual review boards at other research institutions.

Abstract 837: Carbaryl use and cancer incidence in the agricultural health study - An updated analysis

Abstract Background: Carbaryl is a carbamate insecticide with widespread residential and agricultural uses. Previous epidemiologic investigations, including in the Agricultural Health Study (AHS), have suggested potential associations between carbaryl use and cancer risk. In this analysis, we updated a previous AHS evaluation of carbaryl and cancer risk by incorporating additional exposure information and follow-up time. Methods: The AHS is a prospective cohort study of licensed pesticide applicators in North Carolina (NC) and Iowa (IA). Information on lifetime pesticide use, including carbaryl, was reported on questionnaires at enrollment (1993-1997) and follow-up (1999-2005). We evaluated cancer risks associated with ever-use and intensity-weighted lifetime days (IWLD) of carbaryl use, comparing those who reported use to those who did not. For IWLD, carbaryl exposure was categorized (quartiles, tertiles, or the median) among users, depending on the number of cases. Among 52,625 applicators, 8,713 incident cancers were identified from linkages with state cancer registries through 2014 (NC) or 2017 (IA). We used Poisson regression to estimate risk ratios (RR) and 95% confidence intervals (CI), controlling for confounders, and evaluated lagged exposures. Results: Approximately 50% of applicators reported use of carbaryl. Among cancer cases, 53% reported carbaryl use. Increasing IWLD of carbaryl use was associated with stomach cancer (RRT3=2.15, 95% CI: 1.11-4.15, p-trend=0.02), which persisted across lags up to 15 years. Carbaryl users in the highest quartile of IWLD had an increased risk of leukemia (RRQ4=2.12, 95% CI: 1.05-4.29, p-trend=0.08); this risk remained elevated, though attenuated, when exposure was lagged by 5-years (RRQ4=1.86, 95% CI: 0.91-3.81, p-trend=0.14). Ever-use of carbaryl was also suggestively associated with both esophageal (RR=1.45, 95% CI: 0.98-2.14) and tongue (RR=1.87, 95% CI: 0.93-3.74) cancers. Conclusion: This study provides novel evidence of associations between carbaryl use, leukemia, and stomach cancer, as well as suggestive evidence of elevated risks of esophageal and tongue cancers. With more than 7,000 added cancer cases and up to 14 additional years of follow-up time as compared to a previous analysis in the AHS, we were able evaluate cancer sites not previously examined, making this the largest prospective study of carbaryl and cancer risk to date. Our findings highlight the need for additional studies of this widely used insecticide. Citation Format: Patricia A. Erickson, Gabriella Andreotti, Richard Remigio, Christine G. Parks, Dale P. Sandler, Paul S. Albert, Kathryn H. Barry, Jonathan N. Hofmann, Laura E. Beane Freeman. Carbaryl use and cancer incidence in the agricultural health study - An updated analysis [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 837.

Healthcare Utilization Among Infants Covered by Medicaid and Newly Diagnosed With Respiratory Syncytial Virus.

Infants covered by Medicaid have higher respiratory syncytial virus (RSV) hospitalization rates than those with commercial insurance, but findings are limited to the inpatient setting. This birth cohort study describes healthcare encounters for RSV across all settings among infants covered by Medicaid and the Children's Health Insurance Program. Medicaid claims for infants born and residing in Arizona (AZ), California (CA), Florida (FL), Michigan (MI), North Carolina (NC), New York (NY), and Texas (TX) were analyzed for first diagnosis of RSV in 2016-2018 using International Classification of Diseases, Tenth Revision codes. Encounters on the day of first diagnosis were examined by setting in 7 states and by setting and race in CA, FL, and NC. A total of 80 945 infants were diagnosed with RSV in 7 states in 2016-2018. The highest encounter rates for first RSV diagnosis were in the emergency department (ED) in 5 states (11.0-33.4 per 1000 in AZ, CA, FL, MI, and NY) and outpatient setting in 2 states (54.8 and 68.5 per 1000 in TX and NC). Significantly higher outpatient encounter rates were found in CA and NC for White infants compared to non-White infants. In NC, ED encounter rates were significantly higher for non-White infants than White infants, whereas in CA, the rates were comparable. In these 2 states, hospitalization rates were similar across groups. In FL, compared with White infants, non-White infants had significantly higher encounter rates in each setting on the day of first RSV diagnosis. This is the first study to describe the burden of RSV by setting and race. Medicaid infants who are newly diagnosed with RSV have the highest burden in ED and outpatient settings.

Work-Related Stress and Psychological Distress among Law Enforcement Officers: The Carolina Blue Project.

Law enforcement is a stressful occupation that places significant psychological demands on those serving in this role. However, little is known about the severity of work-related stress and psychological distress among law enforcement officers (LEOs) in North Carolina (NC). This cross-sectional study examined the severity of work-related stress and psychological distress among 283 LEOs in NC. The Maslach Burnout Inventory, the Operational Police Stress Questionnaire, the Depression, Anxiety, and Stress Scale, and the Post-Traumatic Stress Disorder (PTSD) Checklist were used to assess burnout, operational police stress, depression, anxiety, stress, and PTSD among LEOs. Descriptive statistics, independent t-tests, Mann-Whitney U tests, one-way ANOVA, and Kruskal-Wallis tests were performed. Rural and male LEOs reported higher burnout levels related to depersonalization (i.e., increased mental distance from one's job) compared with their urban and female counterparts. LEOs exposed to toxic materials or performing patrol duties exhibited higher operational police stress levels than those who did not. Caucasian LEOs exhibited higher depression, anxiety, and stress than their African American counterparts. Rural LEOs and LEOs who were exposed to toxic materials displayed higher levels of PTSD than their counterparts. Our findings highlight the need for increased mental health support and better working environments for LEOs.

Hemadsorption extracorporeal therapy removes cytokines ex vivo in horses.

Plasma cytokine adsorption has shown benefit as an adjunctive therapy in human sepsis but has yet to be investigated in horses. We hypothesized that ex vivo filtration of equine plasma with a novel cytokine adsorption device would significantly reduce concentrations of lipopolysaccharide-stimulated cytokines. We also hypothesized that the device would adsorb medications commonly used to treat sepsis. 8 horses owned by North Carolina State University. Four liters of heparinized whole blood was collected from healthy adult horses (n = 8) and stimulated with lipopolysaccharide (100 ng/mL) for 6 hours (37 °C.) from June 4, 2023, to December 15, 2023. Plasma was filtered through a cytokine adsorption device or sham circuit. Samples were collected at 11 time points for multiplex cytokine analysis. Chemistry analysis was performed before and after filtration. To investigate the impact of the device on medication concentrations, equine plasma containing potassium penicillin, gentamicin, and flunixin meglumine was filtered through the cytokine adsorption device or sham for 6 hours. Drug concentrations before and after filtration were determined by ultra-high-performance liquid chromatography. Prefiltration versus postfiltration sample concentrations were analyzed by Student paired t test using GraphPad Prism 9.0 (P < .05). Filtration of lipopolysaccharide-stimulated equine plasma (n = 8) for 6 hours resulted in significant mean reductions in the cytokines IL-10, IL-5, IL-8, tumor necrosis factor-α (TNF-α), and IL-1β, as well as albumin. Drug concentrations of potassium penicillin, gentamicin, and flunixin meglumine were also significantly reduced by filtration. This work provides proof of concept for further investigation of extracorporeal cytokine adsorption as a potential adjunct treatment for equine sepsis.

Lives Worth Saving: The Impact of Medicaid Expansion on Individuals in Recovery

A quarter of a million North Carolinians admit to experienc­ing opioid use disorder; over 1,000 die each year. Only 1 in 5 receives effective, evidence-based treatment. Medicaid covers treatment and will increase access for members with opioid use disorder, who have been found to fare better than those with private insurance.

NC Medicaid’s Early Experience with a Hospital at Home Program During the Public Health Emergency

A quarter of a million North Carolinians admit to experienc¬ing opioid use disorder; over 1,000 die each year. Only 1 in 5 receives effective, evidence-based treatment. Medicaid covers treatment and will increase access for members with opioid use disorder, who have been found to fare better than those with private insurance.

Risk Factors and Outcomes Associated with Gaps in Care in Children with Congenital Heart Disease.

Adults with congenital heart disease (CHD) benefit from cardiology follow-up at recommended intervals of ≤ 2years. However, benefit for children is less clear given limited studies and unclear current guidelines. We hypothesize there are identifiable risks for gaps in cardiology follow-up in children with CHD and that gaps in follow-up are associated with differences in healthcare utilization. Our cohort included children < 10years old with CHD and a healthcare encounter from 2008 to 2013 at one of four North Carolina (NC) hospitals. We assessed associations between cardiology follow-up and demographics, lesion severity, healthcare access, and educational isolation (EI). We compared healthcare utilization based on follow-up. Overall, 60.4% of 6,969 children received cardiology follow-up within 2years of initial encounter, including 53.1%, 58.1%, and 79.0% of those with valve, shunt, and severe lesions, respectively. Factors associated with gaps in care included increased drive time to a cardiology clinic (Hazard Ratio (HR) 0.92/15-min increase), EI (HR 0.94/0.2-unit increase), lesion severity (HR 0.48 for shunt/valve vs severe), and older age (HR 0.95/month if < 1year old and 0.94/year if > 1year old; p < 0.05). Children with a care gap subsequently had more emergency department (ED) visits (Rate Ratio (RR) 1.59) and fewer inpatient encounters and procedures (RR 0.51, 0.35; p < 0.05). We found novel factors associated with gaps in care for cardiology follow-up in children with CHD and altered health care utilization with a gap. Our findings demonstrate a need to mitigate healthcare barriers and generate clear cardiology follow-up guidelines for children with CHD.

Optimal Mating of Pinus taeda L. Under Different Scenarios Using Differential Evolution Algorithm

Abstract A newly developed software, AgMate, was used to perform optimized mating for monoecious Pinus taeda L. breeding. Using a computational optimization procedure called differential evolution, AgMate was applied under different breeding population sizes scenarios (50, 100, 150, 200, and 250) and candidate contribution scenarios (maximum use of each candidate was set to 1 or 8), to assess its efficiency in maximizing the genetic gain while controlling inbreeding. A population of 962 Pinus taeda parents with a known pedigree from the North Carolina State University Tree Improvement Program was used to optimize objective functions accounting for the coancestry of parents and expected genetic gain and inbreeding of the future progeny. AgMate results were compared with those from another widely used mating software called MateSel. For the proposed mating list of 200 progenies, AgMate resulted in an 83.7% increase in genetic gain compared with the candidate population. There was evidence that AgMate performed similarly to MateSel in managing coancestry and expected genetic gain, but MateSel was superior in avoiding inbreeding in proposed mate pairs. The developed algorithm was computationally efficient in maximizing the objective functions and flexible for practical application in monoecious diploid conifer breeding. AgMate, with its open-source software, free-to-modify algorithm and front-end ShinyApp, is a necessary addition for the advancement of conifer breeding. Study Implications: A dataset from a breeding population of loblolly pine (Pinus taeda L.) was analyzed using an optimal mating software, AgMate (developed by the authors), to optimize the selection, contribution, and mating of candidates simultaneously. The software helps breeders decide on trees to cross and the crossing frequency, such that the trees are unrelated and would result in the best-performing progenies. AgMate is effective in meeting the breeding objectives for monoecious diploid species. The open-source, easy-to-use, and flexible AgMate software, also accessible via a website, is invaluable in helping breeders create optimal matings for future generations, which balances the pursuit of maximizing genetic gain while maintaining genetic diversity.

31 Pastureland Ecology 1 provides hands-on training in grazing management for USDA-NRCS professionals

Abstract Pastureland Ecology 1 is a 2-wk continuing education course taught by cooperating faculty at NC State University for USDA-NRCS Staff. The course was founded in the early 1990s and has been taught 23 times to over 500 participants from all 50 states. Participants primarily include grazing specialists, soil conservationists, and district conservationists. Instruction is split between classroom lectures and hands-on experience with grazing management. Lecture topics include; introduction to pasture ecology, principles of plant growth, determining animal forage needs, pasture design, principles of soil health, principles of grazing management, and grazing management systems for small ruminants, horses and cattle. Small groups of students work with groups of goats, sheep, horses, and beef cattle throughout the course, learning concepts of forage allocation, temporary electric fence, and adaptive management. Popular demonstrations developed over the years include; the reel race, dung beetle buckets, burying underwear to demonstrate soil health, exploring root diversity, and troubleshooting electric fence. Farm tours are conducted to show how concepts being taught are applied on commercial farms. In 2023 there were 29 students from 13 states including VA (4), MD (4), PA (3), OK (4), MI (1), UT (2), TN (3), WV (1), MA (1), OH (1), NM (1), WA (3), and RI (1). Participants had 8.4 ± 1.39 (AVG ± SEM) years of experience with NRCS with a range of 1 to 27 yr. Students self-assessed their understanding of major concepts before and after the course. On a 1 to 5 scale students increased their understanding by an average of 1.05 ± 0.129, averaged over all topics. Topics more familiar to participants had less increase including; Soil Health (0.38), Pasture Condition Evaluation (0.51), and Grazing Management for Beef Cattle (0.79). Less familiar topics had greater increases including Grazing Management for Horses (1.27) and Small Ruminants (1.27), and Temporary Electric Fence (1.43). Pastureland Ecology 1 remains a critical part of the Amazing Grazing Program at NC State University, impacting pasture-based livestock producers across the country. This educational model has proven to be an effective way of teaching the principles of pastureland ecology.

55 Evaluation of feed efficiency and feeding behavior in Katahdin lambs housed in a Vytelle Sense system

Abstract Genetic selection focused on feed efficiency may prove to be valuable in reducing feed consumption costs and animal maintenance requirements without compromising growth. The Vytelle Sense system allows measurement of individual lamb DMI and varying feeding behaviors to identify feed efficient lambs. The aim of this study was to evaluate the impact of sire selection based on fecal egg count estimated breeding value (FEC EBV) on residual feed intake (RFI), gain:feed (G:F), and daily DMI. Katahdin ewes at the Virginia Tech Southwest Virginia Agricultural and Research Center (SWAREC) were mated to Katahdin rams (LowFEC: n = 4; HighFEC: n = 4) to divergently select for FEC EBV in their offspring (LowFEC: n = 36; HighFEC: n = 32). Post-weaning, lambs were fed a complete pellet (14% CP, 76% TDN) ad libitum in the Vytelle Sense system for 58 days. RFI was calculated as the difference between actual and expected DMI. DMI was predicted based on ADG and metabolic body size. Feeding duration per meal and DMI per meal were analyzed to determine feeding behavior. Statistical analyses were performed using PROC Mixed SAS (SAS Institute, Cary, NC). Sire FEC EBV type tended to have an effect on RFI (P = 0.06). Individual sire had a significant effect on RFI, G:F, and daily DMI (P &amp;lt; 0.05). Sex did not impact RFI (P &amp;gt; 0.10). Ram lambs had greater G:F values compared with ewes (0.32 vs. 0.26 kg; P &amp;lt; 0.05). Rams had greater daily DMI compared with ewes (2.37 vs. 1.89 kg/d; P &amp;lt; 0.05). Significance was detected between sires for meal duration (P &amp;lt; 0.05). Ewe lambs exhibited greater meal duration compared with rams (237.3 vs. 203.4 s/meal; P &amp;lt; 0.05). However, ewes had reduced DMI per meal compared with rams (0.29 vs. 0.34 kg/meal; P &amp;lt; 0.05). Individual sire did not have an effect on meal DMI (P &amp;gt; 0.10). To evaluate sire impact on RFI across multiple locations, LowFEC Katahdin rams (n = 2), mentioned previously, were bred to Katahdin ewes at the NC State University Upper Piedmont Research Station (UPRS) and the SWAREC. Genetic connectedness between locations allowed for comparisons of progeny performance. Post-weaning, UPRS lambs were fed a complete pellet (16% CP, 75% TDN) ad libitum in the Vytelle sense system for 45 d. Location did not impact RFI prediction for either sire (P &amp;gt; 0.10). Variation between sires indicates potential sire impact on progeny feed efficiency and feeding behavior. Further studies are needed to evaluate sire RFI trends across multiple locations.

Microbiome of Invasive Tick Species Haemaphysalis Longicornis in North Carolina, USA.

Ticks are one of the most important vectors of human and animal disease worldwide. In addition to pathogens, ticks carry a diverse microbiota of symbiotic and commensal microorganisms. In this study, we used next-generation sequencing (NGS) to survey the microbiomes of Haemaphysalis longicornis (Acari: Ixodidae) at different life stages collected from field populations in North Carolina (NC), USA. Sequence analyses were performed using QIIME2 with the DADA2 plugin and taxonomic assignments using the Greengenes database. Following quality filtering and rarefaction, the bacterial DNA sequences were assigned to 4795 amplicon sequence variants (ASVs) in 105 ticks. A core microbiome of H. longicornis was conserved across all ticks analyzed, and included bacterial taxa: Coxiella, Sphingomonas, Staphylococcus, Acinetobacter, Pseudomonas, Sphingomonadaceae, Actinomycetales, and Sphingobium. Less abundant bacterial taxa, including Rickettsia and Aeromonas, were also identified in some ticks. We discovered some ASVs that are associated with human and animal infections among the identified bacteria. Alpha diversity metrics revealed significant differences in bacterial diversity between life stages. Beta diversity metrics also revealed that bacterial communities across the three life stages were significantly different, suggesting dramatic changes in the microbiome as ticks mature. Based on these results, additional investigation is necessary to determine the significance of the Haemaphysalis longicornis microbiome for animal and human health.

The people behind the papers - Julia Grzymkowski and Nanette Nascone-Yoder.

As the digestive system develops, the gut tube lengthens and convolutes to correctly package the intestine. Intestinal malrotation is a prevalent birth anomaly, but its underlying causes are not well understood. In this new study, Nanette Nascone-Yoder and colleagues show that exposure of Xenopus embryos to atrazine, a widely-used herbicide, can disrupt cellular metabolism in the developing gut tube and lead to intestinal malrotation. We caught up with first author Julia Grzymkowski and corresponding author Nanette Nascone-Yoder, Professor at North Carolina State University, to hear more about the story.

Twenty-five year occupational homicide mortality trends in North Carolina: 1992–2017

IntroductionDetermining industry of decedents and victim–perpetrator relationships is crucial to inform and evaluate occupational homicide prevention strategies. In this study, we examine occupational homicide rates in North Carolina (NC) by...

Naloxone Accessibility by Standing Order in North Carolina Community Pharmacies

BackgroundAccording to a standing order in North Carolina (NC), naloxone can be purchased without a provider prescription. ObjectiveThe objective of this study is to examine whether same-day naloxone accessibility and cost vary by pharmacy type and rurality in NC. MethodsA cross-sectional telephone audit of 202 NC community pharmacies stratified by pharmacy type and county of origin was conducted in March and April 2023. Trained “secret shoppers” enacted a standardized script and recorded whether naloxone was available and its cost. We examined the relationship between out-of-pocket naloxone cost, pharmacy type, and rurality. ResultsNaloxone could be purchased in 53% of the pharmacies contacted; 26% incorrectly noting that naloxone could be filled only with a provider prescription and 21% did not sell naloxone. Naloxone availability by standing order was statistically different by pharmacy type (chain/independent) (χ2 = 20.58, df = 4, P value < 0.001), with a higher frequency of willingness to dispense according to the standing order by chain pharmacies in comparison to independent pharmacies. The average quoted cost for naloxone nasal spray at chain pharmacies was $84.69; the cost was significantly more ($113.54; P < 0.001) at independent pharmacies. Naloxone cost did not significantly differ by pharmacy rurality (F2,136 = 2.38, P = 0.10). ConclusionApproximately half of NC community pharmacies audited dispense naloxone according to the statewide standing order, limiting same-day access to this life-saving medication. Costs were higher at independent pharmacies, which could be due to store-level policies. Future studies should further investigate these cost differences, especially as intranasal naloxone transitions from a prescription only to over-the-counter product.

Abstract B031: Localized cytokine immunotherapy with a novel injectable hydrogel confers immune memory in murine triple negative breast cancer tumors

Abstract Introduction. Triple negative breast cancer (TNBC), representing 10-15% of all diagnosed breast cancer (BC) cases, is the most aggressive BC subtype and has higher recurrence rates and therapy resistance. It typically has an inflamed tumor microenvironment and relatively high tumor infiltrating lymphocyte levels. However, T cells are typically less activated compared to non-invasive BC. Hence, we hypothesize that local delivery of immunotherapeutics to activate and reinvigorate T cells can restore the anti-tumor immune response. To this end, we have developed an injectable delivery medium to effectively localize immune agonists to activate, expand, and reinvigorate T cells at the site of the tumor. Methods. The naturally occurring polysaccharide chitosan was crosslinked to form a novel injectable hydrogel (XCSgel). For in vivo tumor treatment, 2.5 × 105 E0771 murine breast cancer cells were implanted subcutaneously on the right flank of C57BL/6 mice. When tumors grew to be 50-100mm3, 5ug and 20ug of interleukin-12 (IL-12) co-formulated in 50uL of XCSgel was delivered intratumorally, with additional treatment groups of 5ug IL-12 in saline, XCSgel alone, and an untreated control. For re-challenge studies, 2.5 × 105 E0771 murine breast cancer cells were implanted subcutaneously on the left flank of mice, with a naïve cohort as a control. For the second tumor study to reinvigorate T cells in mice with larger tumor burdens, 5 × 105 E0771 murine breast cancer cells were implanted subcutaneously on the right flank of C57BL/6 mice. When tumors reach 100-150mm3, 5ug IL-12 in XCSgel, 20ug IL-10 in XCSgel, 5ug IL-12 + 20ug IL-10 in XCSgel, 5ug IL-12 + 20ug IL-10 in saline, and XCSgel will be delivered intratumorally, with an untreated control. Tumor sizes and Kaplan-Meier survival curves were/will be collected across all cohorts. All experiments involving laboratory animals were approved by the Institutional Animal Care and Use Committee at North Carolina State University. Results and Discussion. In the first tumor study, 5ug IL-12 in XCSgel eliminated tumors in 5/5 mice, while 20ug IL-12 in XCSgel eliminated tumors in 4/5 mice and IL-12 in saline eliminated 2/5 tumors. No tumor delay or elimination was observed in untreated and gel alone groups. Upon re-challenge, 5/5 mice in the 5ug IL-12 in XCSgel remained tumor-free over 40 days post-re-challenge, as did 3/4 of the 20ug in XCSgel and 2/2 in IL-12 saline treatment groups. The second intratumoral study is ongoing. Conclusions. A single injection of IL-12 formulated in a novel injectable hydrogel was found to eliminate tumors and stimulate immune memory in a murine triple negative breast cancer model. Results from localized IL-12 and IL-10 cytokine treatment of tumors with larger tumor burdens are ongoing. Given the ability of this localized immunotherapy to induce systemic antitumor immunity, it deserves further consideration as a neoadjuvant treatment prior to breast conserving surgery.Acknowledgements. This work is supported by the NSF Graduate Research Fellowship. Citation Format: Siena M Mantooth, David A Zaharoff. Localized cytokine immunotherapy with a novel injectable hydrogel confers immune memory in murine triple negative breast cancer tumors [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Advances in Breast Cancer Research; 2023 Oct 19-22; San Diego, California. Philadelphia (PA): AACR; Cancer Res 2024;84(3 Suppl_1):Abstract nr B031.